Optical Trapping and Fast Discrimination of Label-Free Bacteriophages at the Single Virion Level, in Small.

Research

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A: Optofluidic platform and end-fire setup illustration. B: L3 hollow PhC cavity SEM micrograph. C: Resonant mode near-field intensity simulated with the guided mode extension method

We develop an optical tweezer able to operate at the single virion level without any need for virion pre-labelling nor surface bioreceptors and sensitive enough to detect the presence of one, two, three or more virions, here bacteriophages, as well as to distinguish different phages from phenotypically distinct families.

Normalized transmission measurement of an object trapped in the H2 hollow PhC cavity displaying the different regimes of the measurement: Cavity OFF - red, Cavity ON - blue, trapping - green. Insets show the spectral counterparts: in blue, the normalized

N. Villa, E. Tartari, S. Glicenstein, H. de Villiers de la Noue, E. Picard, P. R. Marcoux, M Zelsmann, G Resch, E. Hadji and R. Houdré, accepted in Small

https://doi.org/10.1002/smll.202308814

Submitted on March 12, 2024

Updated on December 5, 2024